We have shown that red blood cell extracellular vesicles (RBCEVs) are the ideal carriers for therapeutic RNAs and they can be produced in a large quantity.
Here, we describe some new development in nucleic acid loading and surface modification of RBCEVs.
First, we describe the delivery of immunomodulatory RNAs (immRNAs) that acts as RIG-I agonists. Intratumoral administration of immRNA in RBCEVs significantly suppressed tumor growth of mammary breast cancer, and induced immune cell infiltration and tumor cell apoptosis mediated by RIG-I activation, turning the ‘cold’ tumors ‘hot’.
Second, we will present some new data on plasmid delivery using RBCEVs via intrathecal injection for Herceptin expression with a potential for brain metastasis treatment.
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